A Manometric Method for Measuring the Activity of the Cl. Welchii Lecithinase and a Description of Certain Properties of This Enzyme
نویسندگان
چکیده
A manometric method has been described for measurement of the activity of Cl. welchii lecithinase. The method depends on displacement of carbon dioxide from a bicarbonate-carbon dioxide-buffered medium by the phosphorylcholine liberated during the enzymatic hydrolysis.
منابع مشابه
A Study of the Competition of Lecithin and Antitoxin for Cl. Welchii Lecithinase
Lecithin has been found to interfere with the combining reaction of Cl. welchii alpha toxin (lecithinase) and its antitoxin. If the lecithinase is first brought into contact with lecithin, and the antitoxin is then added, the antitoxin fails to stop the enzymatic reaction, but gradually decelerates it. If the lecithinase is brought into contact with both lecithin and antitoxin at the same insta...
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ورودعنوان ژورنال:
- The Journal of Experimental Medicine
دوره 85 شماره
صفحات -
تاریخ انتشار 1947